Stage 3
December 16 - December 15 2015


Main objective
The main objective of the project is the development of new multifunctional textile structures, including biologically active compounds extracted from plants, to prevent the spread of infectious diseases such as Borreliosis and other bacterial diseases spread by ticks.

Stage 3 objective – 2015
The main objective of stage 3 was the developemnt of inovative consortia of tick repellent compounds. The specific objectives within the stage, were:
- Pilot level experiments of preparation of new repellent compounds;
- Physical-chemical characterization of natural compounds obtained;
- The evaluation of anti-fungal efficiency;
- Cost-efficient scaling of industrial scale level technology of obtaining natural active compoundes;
- Development and desing of workwear and leisure clothing pattersn, according to market demands;
- Development of patterns library and of graphic standard measurements for clothing;
- Project coordination, technical, administrative and financial management;
- Results dissemination;
- Financial audit.

Stage 3 M5 meeting
Between 05/21-22/2015, Tickotex work meeting was held. The meeting tool place in Istanbul, Turkey, Ozyegin University. At the meeting, Romanian partners involved in the consortia attented, but also representatives sucontracted in the project. The meetings were held during 2 days (May 21 and 22), being focused on presentation of project current work stage, progresses made by each partner, current working stage in each country, future WPs and next steps future planning for each stage.

Stage 3 M10 meeting
Between 10/20-21/2015, a new Tickotex work meeting was held. The meeting tool place in Bucharest, Romania, organized by National Research & Development Institute for Textile & Leather, Bucharest. Both romanian partners involved in the project and representatives of the partners subcontracted within the project attended to the meeting. The meeting aimed disscusions regarding global progress made by each partner, but also work stages that will cover the last year of the project.

GC-MS analysis of the selected essential oils
Extractions were carried out bot in hexane and diethyl ether. The number of compounds identified for each oil varied depending on the solvent used. Components were identified based on comparison of Kovats index (KI), standards co-injection and of MS experimental data comparison with those contained in literature libraries (NIST 02, version 2.62). Alkanes (C5-C24) were used as reference points in obtaining KI. The diluted samples (1/100 in petroleum ether, v/v) of 1µL were injected in splitless mode (ratio 1:10), and all determinations were performed in duplicates and expressed as average.

Table 1. Compusi principali ulei volatil de ienupar

Tabel 2. Compusi principali ulei volatil de rozmarin

Tabel 3. Compusi principali ulei volatil de portocala

Tabel 4. Compusi principali ulei volatil de lavanda

Tabel 5. Compusi principali ulei volatil de eucalipt

The effectiveness of a compound depends on its composition, concentration and type of constituent agents and also the type of ticks on which the compound is tested. The most effective components of the selected oils, are α- terpineol (10.04% in juniper), pinene (25.02% in juniper, 14.41% in rosemary), eucalyptol (40% in rosemary, 91.51% in eucalyptus) and limonene, which is efficient if it is oxidized to carveol.

SEM analysis of textile materials and of selected capsules

Microscopic analysis and identification of nanocapsules (PEG-b-PCL) solutions morphological characteristics (Figure 1), provided by Ozyegin University, were made by Scanning Electron Microscopy (SEM), with a Quanta FEI 200 (Netherlands) electronmicroscope. Morphological analysis was carried out on a GSED detector, ESEM mode. SEM analysis highlighted both shape of particles, degree of agglomeration and size (Figure 1) and also capsules coating of selected textiles.